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(A–B) Laurdan generalized polarization (G.P.) measurements of liposomes derived from inner membrane lipids (IML) and outer membrane lipids (OML). (C–E) <t>TCSPC</t> fluorescence lifetime measurements using Laurdan, TMA-DPH, and DPH probes. These findings demonstrate depth-dependent and asymmetric remodelling of the inner and outer membranes under oxygen limitation. (F–I) Representative Laurdan FLIM images and lifetime distributions of IML- and OML-derived GUVs. Data are mean ± SEM; *p < 0.05, ****p < 0.0001, ns, not significant.
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(A–B) Laurdan generalized polarization (G.P.) measurements of liposomes derived from inner membrane lipids (IML) and outer membrane lipids (OML). (C–E) <t>TCSPC</t> fluorescence lifetime measurements using Laurdan, TMA-DPH, and DPH probes. These findings demonstrate depth-dependent and asymmetric remodelling of the inner and outer membranes under oxygen limitation. (F–I) Representative Laurdan FLIM images and lifetime distributions of IML- and OML-derived GUVs. Data are mean ± SEM; *p < 0.05, ****p < 0.0001, ns, not significant.
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(A–B) Laurdan generalized polarization (G.P.) measurements of liposomes derived from inner membrane lipids (IML) and outer membrane lipids (OML). (C–E) <t>TCSPC</t> fluorescence lifetime measurements using Laurdan, TMA-DPH, and DPH probes. These findings demonstrate depth-dependent and asymmetric remodelling of the inner and outer membranes under oxygen limitation. (F–I) Representative Laurdan FLIM images and lifetime distributions of IML- and OML-derived GUVs. Data are mean ± SEM; *p < 0.05, ****p < 0.0001, ns, not significant.
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(A–B) Laurdan generalized polarization (G.P.) measurements of liposomes derived from inner membrane lipids (IML) and outer membrane lipids (OML). (C–E) <t>TCSPC</t> fluorescence lifetime measurements using Laurdan, TMA-DPH, and DPH probes. These findings demonstrate depth-dependent and asymmetric remodelling of the inner and outer membranes under oxygen limitation. (F–I) Representative Laurdan FLIM images and lifetime distributions of IML- and OML-derived GUVs. Data are mean ± SEM; *p < 0.05, ****p < 0.0001, ns, not significant.
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(A–B) Laurdan generalized polarization (G.P.) measurements of liposomes derived from inner membrane lipids (IML) and outer membrane lipids (OML). (C–E) TCSPC fluorescence lifetime measurements using Laurdan, TMA-DPH, and DPH probes. These findings demonstrate depth-dependent and asymmetric remodelling of the inner and outer membranes under oxygen limitation. (F–I) Representative Laurdan FLIM images and lifetime distributions of IML- and OML-derived GUVs. Data are mean ± SEM; *p < 0.05, ****p < 0.0001, ns, not significant.

Journal: bioRxiv

Article Title: Hypoxia drives asymmetric envelope remodelling to promote membrane adaptation and antibiotic tolerance in mycobacteria

doi: 10.64898/2026.09.12.751103

Figure Lengend Snippet: (A–B) Laurdan generalized polarization (G.P.) measurements of liposomes derived from inner membrane lipids (IML) and outer membrane lipids (OML). (C–E) TCSPC fluorescence lifetime measurements using Laurdan, TMA-DPH, and DPH probes. These findings demonstrate depth-dependent and asymmetric remodelling of the inner and outer membranes under oxygen limitation. (F–I) Representative Laurdan FLIM images and lifetime distributions of IML- and OML-derived GUVs. Data are mean ± SEM; *p < 0.05, ****p < 0.0001, ns, not significant.

Article Snippet: Fluorescence decay was recorded using a DeltaFlex time-correlated single-photon counting (TCSPC) system (HORIBA Scientific).

Techniques: Liposomes, Derivative Assay, Membrane, Fluorescence